|
週次 |
日期 |
單元主題 |
|
第1週 |
2/14 |
Introduction to Proteomics |
|
第2週 |
2/21 |
From genome to proteome |
|
第3週 |
2/28 |
Holiday |
|
第4週 |
3/07 |
Protein isolation and purification |
|
第5週 |
3/14 |
Protein separation and detection |
|
第6週 |
3/21 |
Protein digestion and peptide separation |
|
第7週 |
3/28 |
Mass spectrometry analysis |
|
第8週 |
4/04 |
Holiday |
|
第9週 |
4/11 |
MIDTERM EXAM I (2 hr) 範圍：1-8週內容
報告摘要內容說明 |
|
第10週 |
4/18 |
Protein identification
1.洪正瑜. Proteomic analysis of peach fruit mesocarp softening and chilling injury using difference gel electrophoresis (DIGE). BMC Genomics 2010, 11:43
2.余有勝.Sample Size-Comparable Spectral Library Enhances Data-Independent Acquisition-Based Proteome Coverage of Low-Input Cells. Analytical Chemistry 2021, 93: 17003−17011 |
|
第11週 |
4/25 |
Protein identification Lab
3.陳健笙. Integrative Modeling of a Sin3/HDAC Complex Sub- structure. Cell Reports 2020, 31:107516
4.阮庭皓.Comparative Proteomics Analysis of Bacillus amyloliquefaciens SQR9 Revealed the Key Proteins Involved in in Situ Root Colonization. Journal of Proteome Research 2014, 13:5581-5591 |
|
第12週 |
5/02 |
Quantitative analysis
|
|
第13週 |
5/09 |
Post translational modification(1)- Phospho proteomics
7.楊琇淯. Proteome Alterations in Equine Osteochondrotic Chondrocytes. International Journal of Molecular Sciences 2019, 20:6179
8.劉上源Proteome-wide lysine acetylation identification in developing rice (Oryza sativa) seeds and protein co-modification by acetylation, succinylation, ubiquitination, and phosphorylation.BBA - Proteins and Proteomics 2018, 1866:451–463 |
|
第14週 |
5/16 |
Post translational modification(2)- Glyco proteomics
5.黃詩雅. Quantitative phosphoproteomics reveals the role of wild soybean GsSnRK1 as a metabolic regulator under drought and alkali stresses. Journal of Proteomics 2022, 258:104528
6.劉文智 Serum proteome profiling reveals differentially expressed proteins between subjects with metabolically healthy obesity and nonalcoholic fatty liver disease. Journal of Proteomics 2022, 260:104556
9.張育誠
10.陳偉彬 |
|
第15週 |
5/23 |
Structural proteomics
11.劉律節 Structures of human Nav1.7 channel in complex with auxiliary subunits and animal toxins. Science 2019, l363:1303-1308
12.鄭喆聿 The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity. Nature 2020, 585:569-573 |
|
第16週 |
5/30 |
Bioinformatics tools
13.溫雁荻. Activity-based protein profiling: an efficient approach to study serine hydrolases and their inhibitors in mammals and microbes. RSC Advances 2016, 6:113327
14.蔡文加 Isolation of Arabidopsis extracellular ATP binding proteins byaffinity proteomics and identification of PHOSPHOLIPASE C-LIKE 1 as an extracellular protein essential for fumonisin B1toxicity. Plant Journal 2021, 106:1387–1400 |
|
第17週 |
6/06 |
彈性課程週 |
|
第18週 |
6/13 |
彈性課程週 報告繳交期限為6/13 18:00 |